Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Research Products International Corp TAPS, 5 KG
pKa(25°:C): 8.4. The working pH range in aqueous solution is 7.7-9.1.
Research Products International Corp TES, Sodium Salt, 1 KG
TES (N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid) Sodium Salt is a commonly used buffering agent in life science research. It helps to maintain a stable pH environment in biological experiments, such as cell culture, protein purification, and enzyme assays.
TES Sodium Salt is advantageous due to its ability to resist changes in pH over a wide range of temperatures, making it suitable for various experimental conditions.
Additionally, its low UV absorbance and minimal interference with biological molecules make it a preferred choice in many biochemical applications
Biotium Ready-to-Use Tyramide Amplification Buffer, 1X
Ready-to-use tyramide amplification buffer can be used for tyramide signal amplification with CF Dye Tyramides or other tyramides without the need to add hydrogen peroxide.
Sigma Aldrich Fine Chemicals Biosciences MOPS sodium salt 99.5 titr1KG
MOPS sodium salt 99.5 titr1KG
Cayman Chemical Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium II dichloride complex, Purity: ≥95%, CAS: 36309-88-3, 25 mg
A luminescent probe (absorption λmax: 455 nm, luminescence λmax: 613 nm) widely used for detection and quantitation of oxygen; strongly reduced by molecular oxygen as a result of dynamic quenching; thus oxygen detection can be based on either measurement of intensity or decay time
Sigma Aldrich Fine Chemicals Biosciences HEPES potassium salt | 82207-62-3 | MFCD00043438 | 100g
HEPES potassium salt | Purity: 99.5% | Mol Wt: 273.69 | 82207-62-3 | MFCD00043438 | 100g
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid BioUltra, anhydrous | 60-00-4 | MFCD00003541 | 500g
Ethylenediaminetetraacetic acid BioUltra, anhydrous | Purity: 99% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 500g
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid ACS reagent, 99.4-100.6%, powder | 60-00-4 | MFCD00003541 | 1KG
Ethylenediaminetetraacetic acid ACS reagent, 99.4-100.6%, powder | Purity: 99.4-100.6% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 1KG
Sigma Aldrich Fine Chemicals Biosciences MOPS BioPerformance Certified, suitable for cell culture, >=99.5% (titration) | 1132-61-2 | MFCD00006183 | 6X500G
MOPS BioPerformance Certified, suitable for cell culture, >=99.5% (titration) | Purity: >=99.5% (titration) | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 6X500G
Sigma Aldrich Fine Chemicals Biosciences MOPS BioUltra, for molecular biology, >=99.5% (T) | 1132-61-2 | MFCD00006183 | 100G
MOPS BioUltra, for molecular biology, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 100G
Sigma Aldrich Fine Chemicals Biosciences MOPS BioPerformance Certified, suitable for cell culture, >=99.5% (titration) | 1132-61-2 | MFCD00006183 | 1KG
MOPS BioPerformance Certified, suitable for cell culture, >=99.5% (titration) | Purity: >=99.5% (titration) | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 1KG
Sigma Aldrich Fine Chemicals Biosciences MOPS BioPerformance Certified, suitable for cell culture | 1132-61-2 | MFCD00006183 | 100g
MOPS BioPerformance Certified, suitable for cell culture | Purity: 99.5% | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 100g
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered) - 500ml
Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.
Sigma Aldrich Fine Chemicals Biosciences ACES >=99.0% (titration) | 7365-82-4 | MFCD00008030 | 100G
ACES >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 182.2 | 7365-82-4 | MFCD00008030 | 100G